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polyjet in vitro dna transfection reagent  (SignaGen)

 
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    SignaGen polyjet in vitro dna transfection reagent
    Polyjet In Vitro Dna Transfection Reagent, supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyjet+dna+transfection+reagent/polyjet+dna+transfection+reagent/pmc09481576__41467_2022_33111_MOESM1_ESM-205-22-28
    Average 90 stars, based on 1 article reviews
    polyjet in vitro dna transfection reagent - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Transfection:

    Article Title: Carnitine palmitoyltransferase 1C promotes EMT-associated cisplatin resistance in non-small cell lung cancer cells.
    Article Snippet: .. When the density of the cells reached 80%, the cells were transfected using a PolyJet DNA Transfection Reagent (SignaGen Laboratories, Gaithersburg, MD, USA) according to the manufacturer’s instructions. ..

    Article Title: Carnitine palmitoyltransferase 1C promotes EMT-associated cisplatin resistance in non-small cell lung cancer cells
    Article Snippet: .. When the density of the cells reached 80%, the cells were transfected using a PolyJet DNA Transfection Reagent (SignaGen Laboratories, Gaithersburg, MD, USA) according to the manufacturer’s instructions. ..

    Article Title: The integrated stress response regulates 18S nonfunctional rRNA decay in mammals.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FLAG Sigma-Aldrich Cat# A8592; RRID: AB_439702 HA Sigma-Aldrich Cat# 12013819001; RRID: AB_390917 uS3 Bethyl Laboratories Inc. Cat# A303-840A; RRID: AB_2620191 uS5 Bethyl Laboratories Inc. Cat# A303-794A; RRID: AB_11218192 ATF4 Cell Signaling Technology Cat# 11815; RRID: AB_2616025 b-actin Cell Signaling Technology Cat# 5125; RRID: AB_1903890 GCN2 Cell Signaling Technology Cat# 3302S; RRID: AB_2277617 GCN1 Bethyl Laboratories Inc. Cat# A301-843A; RRID: AB_1264319 eS7 Novus Biologicals LLC Cat# NBP2-94119; RRID: AB_3464049 RNF10 Proteintech Cat# 16936-1-AP; RRID: AB_2181846 RPL11 Bethyl Laboratories Inc. Cat# A303-931A; RRID: AB_2620280 RIOK3 Proteintech Cat# 13593-1-AP; RRID: AB_2178105 Phospho-eIF2⍺ (Ser51) Abcam Cat# ab32157; RRID: AB_732117 eIF2⍺ Cell Signaling Technology Cat# 9722; RRID: AB_2230924 uL10 Proteintech Cat# 11290-2-AP; RRID: AB_2254064 RPLP1 Abcam Inc. Cat# Ab121190; RRID: AB_11129105 Anti-puromycin Sigma-Aldrich Cat# MABE343; RRID: AB_2566826 vinculin Santa Cruz Biotechnologies Cat# sc-73614; RRID: AB_1131294 Chemicals, peptides, and recombinant proteins SUPERaseIn Thermo Fischer Scientific Cat# AM2694 T4 Polynucleotide Kinase New England Biosciences Cat# M0201L T4 RNA Ligase 2, Truncated New England Biosciences Cat# M0242L RNase I Thermo Fischer Scientific Cat# AM2294 Superscript II Thermo Fischer Scientific Cat# 18064014 Superscript III Thermo Fischer Scientific Cat# 56575 GlycoBlue Thermo Fischer Scientific Cat# AM9515 CircLigase ssDNA Ligase Biosearch Technologies Cat# CL4115K Nuclease P1 New England Biosciences Cat# M0660 Maxima Reverse Transcriptase Thermo Fischer Scientific Cat# EP0743 PolyJet DNA transfection reagent SignaGen Laboratories Cat# SL100688 Puromycin Sigma-Aldrich Cat# P7255 Hygromycin B Thermo Fisher Scientific Cat# 10687010 SuperSignal West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat# 34580 SuperSignal West Femto Maximum Sensitivity Substrate Thermo Fisher Scientific Cat# 34095 Critical commercial assays CellTox Green Cytotoxicity Promega Cat# G8741 Deposited data Raw Illumina sequencing data This paper GEO: GSE255784 Raw Nanopore sequencing reads This paper SRA: PRJNA1195343 Proteomics data This paper MassIVE: MSV000096620 Raw data of gels and blots This paper, Mendeley Data Mendeley Data: https://doi.org/10.17632/ mswpwgrbhr.1 (Continued on next page) e1 Molecular Cell 85, 1–15.e1–e8, February 20, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines Flp-In T-REx HeLa Wu et al.28 N/A Flp-In T-REx HeLa GCN2KO This paper N/A Flp-In T-REx HeLa GCN1KO This paper N/A Flp-In T-REx HeLa RNF10KO This paper N/A Flp-In T-REx HeLa RIOK3KO This paper N/A Flp-In T-REx HeLa 18S:WT This paper N/A Flp-In T-REx HeLa 18S:A1824C This paper N/A Oligonucleotides See Table S5 for oligo sequences Recombinant DNA PB-18Sh9H WT This paper N/A PB-18Sh9H A1824C This paper N/A PB-18Sh9tMS2 WT This paper N/A PB-18Sh9tMS2 A1824C This paper N/A pFRT-TO-GCN2 WT This paper N/A pFRT-TO-GCN2 Km This paper N/A pFRT-TO-RIOK3 WT This paper N/A pFRT-TO-RIOK3 DN This paper N/A pFRT-TO-RIOK3 DUIM This paper N/A pFRT-TO-RIOK3 Km This paper N/A Software and algorithms MAGeCK and MAGeCKFlute Li et al.69; Wang et al.70 github.com/liulab-dfci/MAGeCK; github. com/WubingZhang/MAGeCKFlute skewer Jiang et al.71 github.com/relipmoc/skewer STAR Dobin et al.72 github.com/alexdobin/STAR seqtk GitHub github.com/lh3/seqtk GraphPadPrism GraphPad software lnc www.graphpad.com Image J National Institutes of Health imagej.net/ Other Biocomp Gradient Fractionator Biocomp Cat# 100-001

    Article Title: Anti-Inflammatory Activity of Biotransformed Platycodon grandiflorum Root Extracts Containing 3- O -β-D-Glucopyranosyl Platycosides in LPS-Stimulated Alveolar Macrophages, NR8383 Cells
    Article Snippet: The intensity of these protein bands was quantitatively determined using the UN-SCAN-IT gel software version 5.1 (Silk Scientific Inc.). .. For the analysis of NF-κB luciferase activity in NR8383 cells, transient transfection was carried out using PolyJet DNA Transfection Reagent (SignaGen LaboratoriesUSA). .. NR8383 cells were plated at a concentration of 1 × 10 5 cells/well in 12-well plates and incubated for 24 h. Following this incubation, the cells were exposed to plasmid solutions containing 1 μg of NF-κB luciferase reporter plasmids (Addgene, USA) and 0.1 μg of pRL-null vectors.

    Article Title: The primary mechanism for highly potent inhibition of HIV-1 maturation by lenacapavir
    Article Snippet: .. Viruses were generated by transfecting HEK293T cells (10 7 plated in 15 cm plates the previous day) with 30 μg pNL4-3 plasmid DNA using PolyJet DNA transfection reagent (SignaGen Laboratories) according to the manufacturer’s protocol. ..

    Article Title: The primary mechanism for highly potent inhibition of HIV-1 maturation by lenacapavir.
    Article Snippet: .. Transmission electron microscopy of virions Viruses were generated by transfecting HEK293T cells (107 plated in 15 cm plates the previous day) with 30 μg pNL4-3 plasmid DNA using PolyJet DNA transfection reagent (SignaGen Laboratories) according to the manufacturer’s protocol. ..

    Article Title: Axodendritic targeting of TAU and MAP2 and microtubule polarization in iPSC-derived versus SH-SY5Y-derived human neurons
    Article Snippet: .. SHN were transfected using the polymer-based PolyJet DNA transfection reagent (SignaGen) at d7 of differentiation. ..

    Article Title: The primary mechanism for highly potent inhibition of HIV-1 maturation by lenacapavir
    Article Snippet: .. Viruses were generated by transfecting HEK293T cells (10 7 plated in 15 cm plates the previous day) with 30 μg pNL4-3 plasmid DNA using PolyJet DNA transfection reagent (SignaGen Laboratories) according to the manufacturer’s protocol. ..

    Recombinant:

    Article Title: The integrated stress response regulates 18S nonfunctional rRNA decay in mammals.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FLAG Sigma-Aldrich Cat# A8592; RRID: AB_439702 HA Sigma-Aldrich Cat# 12013819001; RRID: AB_390917 uS3 Bethyl Laboratories Inc. Cat# A303-840A; RRID: AB_2620191 uS5 Bethyl Laboratories Inc. Cat# A303-794A; RRID: AB_11218192 ATF4 Cell Signaling Technology Cat# 11815; RRID: AB_2616025 b-actin Cell Signaling Technology Cat# 5125; RRID: AB_1903890 GCN2 Cell Signaling Technology Cat# 3302S; RRID: AB_2277617 GCN1 Bethyl Laboratories Inc. Cat# A301-843A; RRID: AB_1264319 eS7 Novus Biologicals LLC Cat# NBP2-94119; RRID: AB_3464049 RNF10 Proteintech Cat# 16936-1-AP; RRID: AB_2181846 RPL11 Bethyl Laboratories Inc. Cat# A303-931A; RRID: AB_2620280 RIOK3 Proteintech Cat# 13593-1-AP; RRID: AB_2178105 Phospho-eIF2⍺ (Ser51) Abcam Cat# ab32157; RRID: AB_732117 eIF2⍺ Cell Signaling Technology Cat# 9722; RRID: AB_2230924 uL10 Proteintech Cat# 11290-2-AP; RRID: AB_2254064 RPLP1 Abcam Inc. Cat# Ab121190; RRID: AB_11129105 Anti-puromycin Sigma-Aldrich Cat# MABE343; RRID: AB_2566826 vinculin Santa Cruz Biotechnologies Cat# sc-73614; RRID: AB_1131294 Chemicals, peptides, and recombinant proteins SUPERaseIn Thermo Fischer Scientific Cat# AM2694 T4 Polynucleotide Kinase New England Biosciences Cat# M0201L T4 RNA Ligase 2, Truncated New England Biosciences Cat# M0242L RNase I Thermo Fischer Scientific Cat# AM2294 Superscript II Thermo Fischer Scientific Cat# 18064014 Superscript III Thermo Fischer Scientific Cat# 56575 GlycoBlue Thermo Fischer Scientific Cat# AM9515 CircLigase ssDNA Ligase Biosearch Technologies Cat# CL4115K Nuclease P1 New England Biosciences Cat# M0660 Maxima Reverse Transcriptase Thermo Fischer Scientific Cat# EP0743 PolyJet DNA transfection reagent SignaGen Laboratories Cat# SL100688 Puromycin Sigma-Aldrich Cat# P7255 Hygromycin B Thermo Fisher Scientific Cat# 10687010 SuperSignal West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat# 34580 SuperSignal West Femto Maximum Sensitivity Substrate Thermo Fisher Scientific Cat# 34095 Critical commercial assays CellTox Green Cytotoxicity Promega Cat# G8741 Deposited data Raw Illumina sequencing data This paper GEO: GSE255784 Raw Nanopore sequencing reads This paper SRA: PRJNA1195343 Proteomics data This paper MassIVE: MSV000096620 Raw data of gels and blots This paper, Mendeley Data Mendeley Data: https://doi.org/10.17632/ mswpwgrbhr.1 (Continued on next page) e1 Molecular Cell 85, 1–15.e1–e8, February 20, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines Flp-In T-REx HeLa Wu et al.28 N/A Flp-In T-REx HeLa GCN2KO This paper N/A Flp-In T-REx HeLa GCN1KO This paper N/A Flp-In T-REx HeLa RNF10KO This paper N/A Flp-In T-REx HeLa RIOK3KO This paper N/A Flp-In T-REx HeLa 18S:WT This paper N/A Flp-In T-REx HeLa 18S:A1824C This paper N/A Oligonucleotides See Table S5 for oligo sequences Recombinant DNA PB-18Sh9H WT This paper N/A PB-18Sh9H A1824C This paper N/A PB-18Sh9tMS2 WT This paper N/A PB-18Sh9tMS2 A1824C This paper N/A pFRT-TO-GCN2 WT This paper N/A pFRT-TO-GCN2 Km This paper N/A pFRT-TO-RIOK3 WT This paper N/A pFRT-TO-RIOK3 DN This paper N/A pFRT-TO-RIOK3 DUIM This paper N/A pFRT-TO-RIOK3 Km This paper N/A Software and algorithms MAGeCK and MAGeCKFlute Li et al.69; Wang et al.70 github.com/liulab-dfci/MAGeCK; github. com/WubingZhang/MAGeCKFlute skewer Jiang et al.71 github.com/relipmoc/skewer STAR Dobin et al.72 github.com/alexdobin/STAR seqtk GitHub github.com/lh3/seqtk GraphPadPrism GraphPad software lnc www.graphpad.com Image J National Institutes of Health imagej.net/ Other Biocomp Gradient Fractionator Biocomp Cat# 100-001

    Reverse Transcription:

    Article Title: The integrated stress response regulates 18S nonfunctional rRNA decay in mammals.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FLAG Sigma-Aldrich Cat# A8592; RRID: AB_439702 HA Sigma-Aldrich Cat# 12013819001; RRID: AB_390917 uS3 Bethyl Laboratories Inc. Cat# A303-840A; RRID: AB_2620191 uS5 Bethyl Laboratories Inc. Cat# A303-794A; RRID: AB_11218192 ATF4 Cell Signaling Technology Cat# 11815; RRID: AB_2616025 b-actin Cell Signaling Technology Cat# 5125; RRID: AB_1903890 GCN2 Cell Signaling Technology Cat# 3302S; RRID: AB_2277617 GCN1 Bethyl Laboratories Inc. Cat# A301-843A; RRID: AB_1264319 eS7 Novus Biologicals LLC Cat# NBP2-94119; RRID: AB_3464049 RNF10 Proteintech Cat# 16936-1-AP; RRID: AB_2181846 RPL11 Bethyl Laboratories Inc. Cat# A303-931A; RRID: AB_2620280 RIOK3 Proteintech Cat# 13593-1-AP; RRID: AB_2178105 Phospho-eIF2⍺ (Ser51) Abcam Cat# ab32157; RRID: AB_732117 eIF2⍺ Cell Signaling Technology Cat# 9722; RRID: AB_2230924 uL10 Proteintech Cat# 11290-2-AP; RRID: AB_2254064 RPLP1 Abcam Inc. Cat# Ab121190; RRID: AB_11129105 Anti-puromycin Sigma-Aldrich Cat# MABE343; RRID: AB_2566826 vinculin Santa Cruz Biotechnologies Cat# sc-73614; RRID: AB_1131294 Chemicals, peptides, and recombinant proteins SUPERaseIn Thermo Fischer Scientific Cat# AM2694 T4 Polynucleotide Kinase New England Biosciences Cat# M0201L T4 RNA Ligase 2, Truncated New England Biosciences Cat# M0242L RNase I Thermo Fischer Scientific Cat# AM2294 Superscript II Thermo Fischer Scientific Cat# 18064014 Superscript III Thermo Fischer Scientific Cat# 56575 GlycoBlue Thermo Fischer Scientific Cat# AM9515 CircLigase ssDNA Ligase Biosearch Technologies Cat# CL4115K Nuclease P1 New England Biosciences Cat# M0660 Maxima Reverse Transcriptase Thermo Fischer Scientific Cat# EP0743 PolyJet DNA transfection reagent SignaGen Laboratories Cat# SL100688 Puromycin Sigma-Aldrich Cat# P7255 Hygromycin B Thermo Fisher Scientific Cat# 10687010 SuperSignal West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat# 34580 SuperSignal West Femto Maximum Sensitivity Substrate Thermo Fisher Scientific Cat# 34095 Critical commercial assays CellTox Green Cytotoxicity Promega Cat# G8741 Deposited data Raw Illumina sequencing data This paper GEO: GSE255784 Raw Nanopore sequencing reads This paper SRA: PRJNA1195343 Proteomics data This paper MassIVE: MSV000096620 Raw data of gels and blots This paper, Mendeley Data Mendeley Data: https://doi.org/10.17632/ mswpwgrbhr.1 (Continued on next page) e1 Molecular Cell 85, 1–15.e1–e8, February 20, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines Flp-In T-REx HeLa Wu et al.28 N/A Flp-In T-REx HeLa GCN2KO This paper N/A Flp-In T-REx HeLa GCN1KO This paper N/A Flp-In T-REx HeLa RNF10KO This paper N/A Flp-In T-REx HeLa RIOK3KO This paper N/A Flp-In T-REx HeLa 18S:WT This paper N/A Flp-In T-REx HeLa 18S:A1824C This paper N/A Oligonucleotides See Table S5 for oligo sequences Recombinant DNA PB-18Sh9H WT This paper N/A PB-18Sh9H A1824C This paper N/A PB-18Sh9tMS2 WT This paper N/A PB-18Sh9tMS2 A1824C This paper N/A pFRT-TO-GCN2 WT This paper N/A pFRT-TO-GCN2 Km This paper N/A pFRT-TO-RIOK3 WT This paper N/A pFRT-TO-RIOK3 DN This paper N/A pFRT-TO-RIOK3 DUIM This paper N/A pFRT-TO-RIOK3 Km This paper N/A Software and algorithms MAGeCK and MAGeCKFlute Li et al.69; Wang et al.70 github.com/liulab-dfci/MAGeCK; github. com/WubingZhang/MAGeCKFlute skewer Jiang et al.71 github.com/relipmoc/skewer STAR Dobin et al.72 github.com/alexdobin/STAR seqtk GitHub github.com/lh3/seqtk GraphPadPrism GraphPad software lnc www.graphpad.com Image J National Institutes of Health imagej.net/ Other Biocomp Gradient Fractionator Biocomp Cat# 100-001

    CellTox Assay:

    Article Title: The integrated stress response regulates 18S nonfunctional rRNA decay in mammals.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FLAG Sigma-Aldrich Cat# A8592; RRID: AB_439702 HA Sigma-Aldrich Cat# 12013819001; RRID: AB_390917 uS3 Bethyl Laboratories Inc. Cat# A303-840A; RRID: AB_2620191 uS5 Bethyl Laboratories Inc. Cat# A303-794A; RRID: AB_11218192 ATF4 Cell Signaling Technology Cat# 11815; RRID: AB_2616025 b-actin Cell Signaling Technology Cat# 5125; RRID: AB_1903890 GCN2 Cell Signaling Technology Cat# 3302S; RRID: AB_2277617 GCN1 Bethyl Laboratories Inc. Cat# A301-843A; RRID: AB_1264319 eS7 Novus Biologicals LLC Cat# NBP2-94119; RRID: AB_3464049 RNF10 Proteintech Cat# 16936-1-AP; RRID: AB_2181846 RPL11 Bethyl Laboratories Inc. Cat# A303-931A; RRID: AB_2620280 RIOK3 Proteintech Cat# 13593-1-AP; RRID: AB_2178105 Phospho-eIF2⍺ (Ser51) Abcam Cat# ab32157; RRID: AB_732117 eIF2⍺ Cell Signaling Technology Cat# 9722; RRID: AB_2230924 uL10 Proteintech Cat# 11290-2-AP; RRID: AB_2254064 RPLP1 Abcam Inc. Cat# Ab121190; RRID: AB_11129105 Anti-puromycin Sigma-Aldrich Cat# MABE343; RRID: AB_2566826 vinculin Santa Cruz Biotechnologies Cat# sc-73614; RRID: AB_1131294 Chemicals, peptides, and recombinant proteins SUPERaseIn Thermo Fischer Scientific Cat# AM2694 T4 Polynucleotide Kinase New England Biosciences Cat# M0201L T4 RNA Ligase 2, Truncated New England Biosciences Cat# M0242L RNase I Thermo Fischer Scientific Cat# AM2294 Superscript II Thermo Fischer Scientific Cat# 18064014 Superscript III Thermo Fischer Scientific Cat# 56575 GlycoBlue Thermo Fischer Scientific Cat# AM9515 CircLigase ssDNA Ligase Biosearch Technologies Cat# CL4115K Nuclease P1 New England Biosciences Cat# M0660 Maxima Reverse Transcriptase Thermo Fischer Scientific Cat# EP0743 PolyJet DNA transfection reagent SignaGen Laboratories Cat# SL100688 Puromycin Sigma-Aldrich Cat# P7255 Hygromycin B Thermo Fisher Scientific Cat# 10687010 SuperSignal West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat# 34580 SuperSignal West Femto Maximum Sensitivity Substrate Thermo Fisher Scientific Cat# 34095 Critical commercial assays CellTox Green Cytotoxicity Promega Cat# G8741 Deposited data Raw Illumina sequencing data This paper GEO: GSE255784 Raw Nanopore sequencing reads This paper SRA: PRJNA1195343 Proteomics data This paper MassIVE: MSV000096620 Raw data of gels and blots This paper, Mendeley Data Mendeley Data: https://doi.org/10.17632/ mswpwgrbhr.1 (Continued on next page) e1 Molecular Cell 85, 1–15.e1–e8, February 20, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines Flp-In T-REx HeLa Wu et al.28 N/A Flp-In T-REx HeLa GCN2KO This paper N/A Flp-In T-REx HeLa GCN1KO This paper N/A Flp-In T-REx HeLa RNF10KO This paper N/A Flp-In T-REx HeLa RIOK3KO This paper N/A Flp-In T-REx HeLa 18S:WT This paper N/A Flp-In T-REx HeLa 18S:A1824C This paper N/A Oligonucleotides See Table S5 for oligo sequences Recombinant DNA PB-18Sh9H WT This paper N/A PB-18Sh9H A1824C This paper N/A PB-18Sh9tMS2 WT This paper N/A PB-18Sh9tMS2 A1824C This paper N/A pFRT-TO-GCN2 WT This paper N/A pFRT-TO-GCN2 Km This paper N/A pFRT-TO-RIOK3 WT This paper N/A pFRT-TO-RIOK3 DN This paper N/A pFRT-TO-RIOK3 DUIM This paper N/A pFRT-TO-RIOK3 Km This paper N/A Software and algorithms MAGeCK and MAGeCKFlute Li et al.69; Wang et al.70 github.com/liulab-dfci/MAGeCK; github. com/WubingZhang/MAGeCKFlute skewer Jiang et al.71 github.com/relipmoc/skewer STAR Dobin et al.72 github.com/alexdobin/STAR seqtk GitHub github.com/lh3/seqtk GraphPadPrism GraphPad software lnc www.graphpad.com Image J National Institutes of Health imagej.net/ Other Biocomp Gradient Fractionator Biocomp Cat# 100-001

    Nanopore Sequencing:

    Article Title: The integrated stress response regulates 18S nonfunctional rRNA decay in mammals.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FLAG Sigma-Aldrich Cat# A8592; RRID: AB_439702 HA Sigma-Aldrich Cat# 12013819001; RRID: AB_390917 uS3 Bethyl Laboratories Inc. Cat# A303-840A; RRID: AB_2620191 uS5 Bethyl Laboratories Inc. Cat# A303-794A; RRID: AB_11218192 ATF4 Cell Signaling Technology Cat# 11815; RRID: AB_2616025 b-actin Cell Signaling Technology Cat# 5125; RRID: AB_1903890 GCN2 Cell Signaling Technology Cat# 3302S; RRID: AB_2277617 GCN1 Bethyl Laboratories Inc. Cat# A301-843A; RRID: AB_1264319 eS7 Novus Biologicals LLC Cat# NBP2-94119; RRID: AB_3464049 RNF10 Proteintech Cat# 16936-1-AP; RRID: AB_2181846 RPL11 Bethyl Laboratories Inc. Cat# A303-931A; RRID: AB_2620280 RIOK3 Proteintech Cat# 13593-1-AP; RRID: AB_2178105 Phospho-eIF2⍺ (Ser51) Abcam Cat# ab32157; RRID: AB_732117 eIF2⍺ Cell Signaling Technology Cat# 9722; RRID: AB_2230924 uL10 Proteintech Cat# 11290-2-AP; RRID: AB_2254064 RPLP1 Abcam Inc. Cat# Ab121190; RRID: AB_11129105 Anti-puromycin Sigma-Aldrich Cat# MABE343; RRID: AB_2566826 vinculin Santa Cruz Biotechnologies Cat# sc-73614; RRID: AB_1131294 Chemicals, peptides, and recombinant proteins SUPERaseIn Thermo Fischer Scientific Cat# AM2694 T4 Polynucleotide Kinase New England Biosciences Cat# M0201L T4 RNA Ligase 2, Truncated New England Biosciences Cat# M0242L RNase I Thermo Fischer Scientific Cat# AM2294 Superscript II Thermo Fischer Scientific Cat# 18064014 Superscript III Thermo Fischer Scientific Cat# 56575 GlycoBlue Thermo Fischer Scientific Cat# AM9515 CircLigase ssDNA Ligase Biosearch Technologies Cat# CL4115K Nuclease P1 New England Biosciences Cat# M0660 Maxima Reverse Transcriptase Thermo Fischer Scientific Cat# EP0743 PolyJet DNA transfection reagent SignaGen Laboratories Cat# SL100688 Puromycin Sigma-Aldrich Cat# P7255 Hygromycin B Thermo Fisher Scientific Cat# 10687010 SuperSignal West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific Cat# 34580 SuperSignal West Femto Maximum Sensitivity Substrate Thermo Fisher Scientific Cat# 34095 Critical commercial assays CellTox Green Cytotoxicity Promega Cat# G8741 Deposited data Raw Illumina sequencing data This paper GEO: GSE255784 Raw Nanopore sequencing reads This paper SRA: PRJNA1195343 Proteomics data This paper MassIVE: MSV000096620 Raw data of gels and blots This paper, Mendeley Data Mendeley Data: https://doi.org/10.17632/ mswpwgrbhr.1 (Continued on next page) e1 Molecular Cell 85, 1–15.e1–e8, February 20, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines Flp-In T-REx HeLa Wu et al.28 N/A Flp-In T-REx HeLa GCN2KO This paper N/A Flp-In T-REx HeLa GCN1KO This paper N/A Flp-In T-REx HeLa RNF10KO This paper N/A Flp-In T-REx HeLa RIOK3KO This paper N/A Flp-In T-REx HeLa 18S:WT This paper N/A Flp-In T-REx HeLa 18S:A1824C This paper N/A Oligonucleotides See Table S5 for oligo sequences Recombinant DNA PB-18Sh9H WT This paper N/A PB-18Sh9H A1824C This paper N/A PB-18Sh9tMS2 WT This paper N/A PB-18Sh9tMS2 A1824C This paper N/A pFRT-TO-GCN2 WT This paper N/A pFRT-TO-GCN2 Km This paper N/A pFRT-TO-RIOK3 WT This paper N/A pFRT-TO-RIOK3 DN This paper N/A pFRT-TO-RIOK3 DUIM This paper N/A pFRT-TO-RIOK3 Km This paper N/A Software and algorithms MAGeCK and MAGeCKFlute Li et al.69; Wang et al.70 github.com/liulab-dfci/MAGeCK; github. com/WubingZhang/MAGeCKFlute skewer Jiang et al.71 github.com/relipmoc/skewer STAR Dobin et al.72 github.com/alexdobin/STAR seqtk GitHub github.com/lh3/seqtk GraphPadPrism GraphPad software lnc www.graphpad.com Image J National Institutes of Health imagej.net/ Other Biocomp Gradient Fractionator Biocomp Cat# 100-001

    Luciferase:

    Article Title: Anti-Inflammatory Activity of Biotransformed Platycodon grandiflorum Root Extracts Containing 3- O -β-D-Glucopyranosyl Platycosides in LPS-Stimulated Alveolar Macrophages, NR8383 Cells
    Article Snippet: The intensity of these protein bands was quantitatively determined using the UN-SCAN-IT gel software version 5.1 (Silk Scientific Inc.). .. For the analysis of NF-κB luciferase activity in NR8383 cells, transient transfection was carried out using PolyJet DNA Transfection Reagent (SignaGen LaboratoriesUSA). .. NR8383 cells were plated at a concentration of 1 × 10 5 cells/well in 12-well plates and incubated for 24 h. Following this incubation, the cells were exposed to plasmid solutions containing 1 μg of NF-κB luciferase reporter plasmids (Addgene, USA) and 0.1 μg of pRL-null vectors.

    Activity Assay:

    Article Title: Anti-Inflammatory Activity of Biotransformed Platycodon grandiflorum Root Extracts Containing 3- O -β-D-Glucopyranosyl Platycosides in LPS-Stimulated Alveolar Macrophages, NR8383 Cells
    Article Snippet: The intensity of these protein bands was quantitatively determined using the UN-SCAN-IT gel software version 5.1 (Silk Scientific Inc.). .. For the analysis of NF-κB luciferase activity in NR8383 cells, transient transfection was carried out using PolyJet DNA Transfection Reagent (SignaGen LaboratoriesUSA). .. NR8383 cells were plated at a concentration of 1 × 10 5 cells/well in 12-well plates and incubated for 24 h. Following this incubation, the cells were exposed to plasmid solutions containing 1 μg of NF-κB luciferase reporter plasmids (Addgene, USA) and 0.1 μg of pRL-null vectors.

    Generated:

    Article Title: The primary mechanism for highly potent inhibition of HIV-1 maturation by lenacapavir
    Article Snippet: .. Viruses were generated by transfecting HEK293T cells (10 7 plated in 15 cm plates the previous day) with 30 μg pNL4-3 plasmid DNA using PolyJet DNA transfection reagent (SignaGen Laboratories) according to the manufacturer’s protocol. ..

    Article Title: The primary mechanism for highly potent inhibition of HIV-1 maturation by lenacapavir.
    Article Snippet: .. Transmission electron microscopy of virions Viruses were generated by transfecting HEK293T cells (107 plated in 15 cm plates the previous day) with 30 μg pNL4-3 plasmid DNA using PolyJet DNA transfection reagent (SignaGen Laboratories) according to the manufacturer’s protocol. ..

    Article Title: The primary mechanism for highly potent inhibition of HIV-1 maturation by lenacapavir
    Article Snippet: .. Viruses were generated by transfecting HEK293T cells (10 7 plated in 15 cm plates the previous day) with 30 μg pNL4-3 plasmid DNA using PolyJet DNA transfection reagent (SignaGen Laboratories) according to the manufacturer’s protocol. ..

    Plasmid Preparation:

    Article Title: The primary mechanism for highly potent inhibition of HIV-1 maturation by lenacapavir
    Article Snippet: .. Viruses were generated by transfecting HEK293T cells (10 7 plated in 15 cm plates the previous day) with 30 μg pNL4-3 plasmid DNA using PolyJet DNA transfection reagent (SignaGen Laboratories) according to the manufacturer’s protocol. ..

    Article Title: The primary mechanism for highly potent inhibition of HIV-1 maturation by lenacapavir.
    Article Snippet: .. Transmission electron microscopy of virions Viruses were generated by transfecting HEK293T cells (107 plated in 15 cm plates the previous day) with 30 μg pNL4-3 plasmid DNA using PolyJet DNA transfection reagent (SignaGen Laboratories) according to the manufacturer’s protocol. ..

    Article Title: The primary mechanism for highly potent inhibition of HIV-1 maturation by lenacapavir
    Article Snippet: .. Viruses were generated by transfecting HEK293T cells (10 7 plated in 15 cm plates the previous day) with 30 μg pNL4-3 plasmid DNA using PolyJet DNA transfection reagent (SignaGen Laboratories) according to the manufacturer’s protocol. ..

    Transmission Assay:

    Article Title: The primary mechanism for highly potent inhibition of HIV-1 maturation by lenacapavir.
    Article Snippet: .. Transmission electron microscopy of virions Viruses were generated by transfecting HEK293T cells (107 plated in 15 cm plates the previous day) with 30 μg pNL4-3 plasmid DNA using PolyJet DNA transfection reagent (SignaGen Laboratories) according to the manufacturer’s protocol. ..

    Electron Microscopy:

    Article Title: The primary mechanism for highly potent inhibition of HIV-1 maturation by lenacapavir.
    Article Snippet: .. Transmission electron microscopy of virions Viruses were generated by transfecting HEK293T cells (107 plated in 15 cm plates the previous day) with 30 μg pNL4-3 plasmid DNA using PolyJet DNA transfection reagent (SignaGen Laboratories) according to the manufacturer’s protocol. ..

    Polymer:

    Article Title: Axodendritic targeting of TAU and MAP2 and microtubule polarization in iPSC-derived versus SH-SY5Y-derived human neurons
    Article Snippet: .. SHN were transfected using the polymer-based PolyJet DNA transfection reagent (SignaGen) at d7 of differentiation. ..



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